4CVM
PaMurF in complex with AMP-PNP and UDP-MurNAc-tripeptide (mDAP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.540, 86.050, 88.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.610 - 2.060 |
| R-factor | 0.18435 |
| Rwork | 0.182 |
| R-free | 0.22442 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.773 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.610 | 2.110 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.160 | 1.170 |
| Number of reflections | 28306 | |
| <I/σ(I)> | 12.6 | 3.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 12.8 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MG FORMATE, 20 % PEG3350 |
