4CVK
PaMurF in complex with UDP-MurNAc-tripeptide (mDAP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.810, 87.290, 92.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.040 - 1.920 |
| R-factor | 0.19717 |
| Rwork | 0.196 |
| R-free | 0.22562 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gg4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.558 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.040 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 36216 | |
| <I/σ(I)> | 13.2 | 2.4 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MG FORMATE, 20 % PEG3350 |
