4CVD
Crystal structure of the central repeat of cell wall binding module of Cpl7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-23 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 |
| Unit cell lengths | 50.400, 50.400, 28.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.200 - 1.666 |
| R-factor | 0.1721 |
| Rwork | 0.170 |
| R-free | 0.21890 |
| Structure solution method | AB INITIO PHASING |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.197 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALE (CCP4) |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.540 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.050 | 0.180 |
| Number of reflections | 4935 | |
| <I/σ(I)> | 10.3 | 4.3 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 10.7 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 1.3 M TRI-SODIUM CITRATE, HEGA-8 AS ADITIVE, VAPOR DIFFUSION, HANGING DROP, pH 5.5 |
