4CVB
Crystal structure of quinone-dependent alcohol dehydrogenase from Pseudogluconobacter saccharoketogenenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2009-02-02 |
Detector | MACSCIENCE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 127.950, 87.190, 57.100 |
Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
Resolution | 27.950 - 1.720 |
R-factor | 0.12872 |
Rwork | 0.128 |
R-free | 0.14858 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1KB0 AND 1YIQ |
RMSD bond length | 0.010 |
RMSD bond angle | 1.357 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.500 | 1.840 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.080 | 0.230 |
Number of reflections | 59031 | |
<I/σ(I)> | 11.4 | 4.3 |
Completeness [%] | 88.9 | 31.3 |
Redundancy | 3.3 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 18-21% PEG550 MME, 20 MM ZN(AC)2, 100 MM PCB BUFFER PH 6-7 |