4CUK
Structure of Salmonella D-Lactate Dehydrogenase in complex with NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.350, 60.700, 130.390 |
| Unit cell angles | 90.00, 106.92, 90.00 |
Refinement procedure
| Resolution | 105.570 - 2.180 |
| R-factor | 0.17918 |
| Rwork | 0.177 |
| R-free | 0.22396 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO STRUCTURE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.848 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.220 | 2.300 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.070 | 0.290 |
| Number of reflections | 86835 | |
| <I/σ(I)> | 11.5 | 3.8 |
| Completeness [%] | 96.1 | 97.3 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M [ IMIDAZOLE; MES (ACID) ] PH 6.5; 0.06M [ MGCL2; CACL2 ]; 30% [ GLYCEROL; PEG 4K ] |
