4CUG
Rhodothermus marinus YCFD-like ribosomal protein L16 Arginyl hydroxylase in complex substrate fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.220, 117.470, 120.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.740 - 2.960 |
| R-factor | 0.2112 |
| Rwork | 0.210 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ccl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.775 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.740 | 3.040 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Rmerge | 0.050 | 0.770 |
| Number of reflections | 29641 | |
| <I/σ(I)> | 19.4 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS-HCL PH8.5, 1MM MNCL2, 1MM OGA, 2MM L16 PEPTIDE, 0.66MM PROTEIN, 0.1M AMMONIUM DIHYDROGEN PHOSPHATE, 45% (V/V) METHYL-2-4-PENTANEDIOL, ROOM TEMPERATURE |
