4CUC
Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 117.340, 117.340, 219.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 117.340 - 2.200 |
R-factor | 0.15532 |
Rwork | 0.153 |
R-free | 0.19127 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.452 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.490 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.200 | 0.640 |
Number of reflections | 78624 | |
<I/σ(I)> | 10.1 | 5.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.8 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |