4CUB
Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 70.530, 70.530, 161.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.620 - 2.100 |
R-factor | 0.19242 |
Rwork | 0.190 |
R-free | 0.24718 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.363 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.150 | 0.410 |
Number of reflections | 24686 | |
<I/σ(I)> | 9.5 | 4.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 |