4CU5
C-terminal domain of endolysin from phage CD27L is a trigger and release factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.301, 82.069, 83.836 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.100 - 2.240 |
| R-factor | 0.18892 |
| Rwork | 0.186 |
| R-free | 0.24741 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.765 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.370 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.130 | 0.600 |
| Number of reflections | 24189 | |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 97.9 | 93.8 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10 % PEG 20K AND 20 MM TRIS PH 8.0 |
