4CSU
Cryo-EM structures of the 50S ribosome subunit bound with ObgE
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI EAGLE (4k x 4k) |
| Number of Images | 2000 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.5 |
| Software | MDFF,RELION |
| Symmetry | C1 |
