4CSP
Structure of the F306C mutant of nitrite reductase from Achromobacter xylosoxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 |
| Unit cell lengths | 89.990, 89.990, 289.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.470 - 1.700 |
| R-factor | 0.18279 |
| Rwork | 0.181 |
| R-free | 0.21482 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oe1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.470 | 1.790 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 97508 | |
| <I/σ(I)> | 11.6 | 2 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
