4CSE
PIH N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-05 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.220, 67.730, 104.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.759 - 3.300 |
| R-factor | 0.2176 |
| Rwork | 0.213 |
| R-free | 0.30550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ckt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.298 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.760 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.170 | 0.600 |
| Number of reflections | 5693 | |
| <I/σ(I)> | 12.8 | 4.7 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 11.7 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.15M SODIUM POTASSIUM PHOSPHATE, 20% PEG 3350, 0.1M BIS-TRIS PH 6.5 |
