4CS8
Crystal structure of the asymmetric human metapneumovirus M2-1 tetramer, form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-12 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.980, 93.420, 85.210 |
Unit cell angles | 90.00, 95.44, 90.00 |
Refinement procedure
Resolution | 30.750 - 2.100 |
R-factor | 0.1938 |
Rwork | 0.192 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cs7 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.810 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | 0.830 |
Number of reflections | 45494 | |
<I/σ(I)> | 23.1 | 2 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 11.8 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 28 % W/V POLYETHYLENE GLYCOL MONOMETHYL ETHER 2000, 0.100 M BIS-TRIS PH 6.5 |