4CS7
Crystal structure of the asymmetric human metapneumovirus M2-1 tetramer, form 1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.2828, 1.2802 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.150, 92.670, 82.830 |
| Unit cell angles | 90.00, 94.48, 90.00 |
Refinement procedure
| Resolution | 33.130 - 2.470 |
| R-factor | 0.2348 |
| Rwork | 0.233 |
| R-free | 0.26150 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.130 | 2.530 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.080 | 1.340 |
| Number of reflections | 27190 | |
| <I/σ(I)> | 12.2 | 1.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 28 % W/V POLYETHYLENE GLYCOL MONOMETHYL ETHER 2000, 0.100 M BIS-TRIS PH 6.5 |
