4CRZ
Direct visualisation of strain-induced protein prost-translational modification
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2013-12-03 |
Detector | RIGAKU R-AXIS IV |
Spacegroup name | I 4 |
Unit cell lengths | 86.280, 86.280, 80.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.520 - 1.700 |
R-factor | 0.14611 |
Rwork | 0.145 |
R-free | 0.17519 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4azd |
RMSD bond length | 0.023 |
RMSD bond angle | 2.333 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.700 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.040 | 0.400 |
Number of reflections | 32244 | |
<I/σ(I)> | 13.3 | 2.2 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 20% (W/V) POLYETHYLENE GLYCOL (PEG) 3350, 0.1 M BIS-TRIS PROPANE PH 7.4, 0.2 M POTASSIUM THIOCYANATE |