4CRX
ASYMMETRIC DNA-BENDING IN THE CRE-LOXP SITE-SPECIFIC RECOMBINATION SYNAPSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-12 |
| Detector | ADSC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.800, 122.100, 181.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.300 - 2.200 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1crx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.062 * | 0.261 * |
| Number of reflections | 55807 | |
| <I/σ(I)> | 6.9 | 0.7 |
| Completeness [%] | 91.7 | 75.6 |
| Redundancy | 2.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 * | 18 * | 20MM ACETATE PH 5.0 35% MPD 20MM CACL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.013 (mM) | |
| 2 | 1 | drop | loxS site | 0.01 (mM) | |
| 3 | 1 | drop | sodium acetate | 20 (mM) | |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | MPD | 25 (%(v/v)) | |
| 7 | 1 | reservoir | sodium acetate | 20 (mM) | |
| 8 | 1 | reservoir | 20 (mM) | ||
| 9 | 1 | reservoir | MPD | 35 (%) |
