4CRI
Crystal Structure of 53BP1 tandem tudor domains in complex with methylated K810 Rb peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 105.789, 105.789, 156.153 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.510 - 2.350 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lh0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.329 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.050 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.080 | 0.840 |
| Number of reflections | 22195 | |
| <I/σ(I)> | 17.8 | 2.2 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 13.7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 5%(W/V) PEG10K. 0.15M MAGNESIUM CHLORIDE, 0.1M TRIS PH 7.5 |
