4CRI
Crystal Structure of 53BP1 tandem tudor domains in complex with methylated K810 Rb peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-30 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 105.789, 105.789, 156.153 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.510 - 2.350 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.22950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lh0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.329 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.050 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.080 | 0.840 |
Number of reflections | 22195 | |
<I/σ(I)> | 17.8 | 2.2 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 13.7 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 5%(W/V) PEG10K. 0.15M MAGNESIUM CHLORIDE, 0.1M TRIS PH 7.5 |