4CRH

Crystal structure of the BTB-T1 domain of human SHKBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-09
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 65
Unit cell lengths	71.290, 71.290, 38.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.645 - 1.720
R-factor	0.1651
Rwork	0.164
R-free	0.19280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3drx
RMSD bond length	0.008
RMSD bond angle	1.211
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.410	1.760
High resolution limit [Å]	1.720	1.720
Rmerge	0.050	0.690
Number of reflections	11971
<I/σ(I)>	21.7	2.4
Completeness [%]	99.8	100
Redundancy	5.6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.4		17% PEG 2000, MME 0.1M TRIS PH 8.4, 0.15M TMAO