4CR7
Crystal structure of the N-acetyl-D-mannosamine dehydrogenase with n-acetylmannosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2011-06-08 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 1 |
| Unit cell lengths | 99.390, 100.120, 111.600 |
| Unit cell angles | 67.43, 89.75, 72.46 |
Refinement procedure
| Resolution | 15.000 - 2.150 |
| R-factor | 0.20225 |
| Rwork | 0.201 |
| R-free | 0.23102 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d1y |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.333 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 189715 | |
| <I/σ(I)> | 5.2 | 1.7 |
| Completeness [%] | 96.9 | 97.8 |
| Redundancy | 2.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M HEPES, PH 7.5 AND 30% (W/V) PEG [POLY(ETHYLENE GLYCOL)] 300 |
