4CR6
Crystal structure of the N-acetyl-D-mannosamine dehydrogenase without substrates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2011-05-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.255, 59.062, 74.206 |
| Unit cell angles | 81.13, 73.29, 79.25 |
Refinement procedure
| Resolution | 12.000 - 1.900 |
| R-factor | 0.20966 |
| Rwork | 0.209 |
| R-free | 0.22844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d1y |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.347 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.170 |
| Number of reflections | 63579 | |
| <I/σ(I)> | 6.2 | 3.9 |
| Completeness [%] | 90.5 | 92.7 |
| Redundancy | 1.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M HEPES, PH 7.5 AND 30% (W/V) PEG [POLY(ETHYLENE GLYCOL)] 300 |
