4CQZ

Crystal Structure of H5 (VN1194) Gln196Arg Mutant Haemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	H 3 2
Unit cell lengths	101.149, 101.149, 447.887
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	149.300 - 2.700
R-factor	0.21899
Rwork	0.217
R-free	0.25225
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bgw
RMSD bond length	0.005
RMSD bond angle	0.982
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0046)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.650	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.080	0.430
Number of reflections	24883
<I/σ(I)>	11.7	1.9
Completeness [%]	99.9	99.4
Redundancy	5.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M HEPES/MOPS PH 7.0, 0.05 M MGCL2, 28-30% PEG 550 MME, SEEDED WITH CRUSHED WILD-TYPE VN1194 HA CRYSTALS.