4CQW
H5 (tyTy) Del133/Ile155Thr Mutant Haemagglutinin in Complex with Avian Receptor Analogue 3'SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.635, 117.552, 101.160 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 101.060 - 2.300 |
| R-factor | 0.1977 |
| Rwork | 0.196 |
| R-free | 0.23788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bgz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.071 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.060 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.660 |
| Number of reflections | 89337 | |
| <I/σ(I)> | 11.9 | 2 |
| Completeness [%] | 98.3 | 98.7 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BIS-TRIS PROPANE PH 7.5, 0.05 - 0.15 M K/NAPO4 (PH 7.0), 15-18% PEG 3350 |
