4CQV

Crystal structure of H5 (tyTy) Del133/Ile155Thr Mutant Haemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	87.407, 117.251, 101.003
Unit cell angles	90.00, 92.52, 90.00

Refinement procedure

Resolution	70.130 - 2.860
R-factor	0.19778
Rwork	0.195
R-free	0.24444
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bgz
RMSD bond length	0.006
RMSD bond angle	1.170
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.030	3.010
High resolution limit [Å]	2.860	2.860
Rmerge	0.080	0.520
Number of reflections	46268
<I/σ(I)>	8.8	2
Completeness [%]	98.3	99
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				BIS-TRIS PROPANE PH 7.5, 0.05 - 0.15 M K/NAPO4 (PH 7.0), 15-18% PEG 3350