4CQ4
C-terminal fragment of Af1503-sol: transmembrane receptor Af1503 from Archaeoglobus fulgidus engineered for solubility
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.069, 48.037, 95.187 |
| Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
| Resolution | 37.670 - 1.700 |
| R-factor | 0.19538 |
| Rwork | 0.193 |
| R-free | 0.24303 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zrx |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.852 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 43434 | |
| <I/σ(I)> | 11.2 | 2.17 |
| Completeness [%] | 99.2 | 97.1 |
| Redundancy | 3.79 | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% (W/V) PEG 3350, 100 MM HEPES, PH 6.5 |
