4CP4

CRYSTAL STRUCTURE OF THE CYTOCHROME P450-CAM ACTIVE SITE MUTANT THR252ALA

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	108.670, 103.900, 36.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.100
R-factor	0.164
RMSD bond length	0.024
RMSD bond angle	0.041 *
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.087 *
Number of reflections	56897 *
Completeness [%]		55 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	0.05 (ml)
2	1	1	ammonium sulfate	0.05 (ml)
3	1	1	potassium phosphate	50 (mM)
4	1	1		250 (mM)
5	1	1	dithiotheitol	50 (mM)