4CO6

Crystal structure of the Nipah virus RNA free nucleoprotein- phosphoprotein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-26
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	82.890, 98.960, 156.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.189 - 2.498
R-factor	0.1952
Rwork	0.192
R-free	0.25930
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.151
Data reduction software	XDS
Data scaling software	XDS
Phasing software	HKL2MAP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.460
Number of reflections	85866
<I/σ(I)>	10.5	2.1
Completeness [%]	99.3	97.9
Redundancy	3.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				MICROSEEDING CONDITIONS 0.2M KBR, 16-20% PEG 3350;