4CO5

Structure of PII signaling protein GlnZ from Azospirillum brasilense in complex with tartrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-24
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	H 3 2
Unit cell lengths	89.700, 89.700, 108.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.450 - 1.800
R-factor	0.1967
Rwork	0.195
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cny
RMSD bond length	0.006
RMSD bond angle	0.938
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.450	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.080	0.420
Number of reflections	15755
<I/σ(I)>	18.8	4.7
Completeness [%]	99.9	100
Redundancy	10.84	10.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2 MM 2OG, 0.1 M HEPES PH 7.5, 0.8 M K/NA TARTRATE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K