4CO4

Structure of PII signaling protein GlnZ from Azospirillum brasilense in complex with adenosine triphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-05-03
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	53.681, 58.950, 90.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.155 - 1.500
R-factor	0.1681
Rwork	0.166
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cny
RMSD bond length	0.005
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.160	1.600
High resolution limit [Å]	1.500	1.500
Rmerge	0.180	0.690
Number of reflections	45979
<I/σ(I)>	8.1	2.5
Completeness [%]	98.5	96.7
Redundancy	6.73	6.37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	15 MM ATP, 0.2 M NACL, 0.1 M PHOSPHATE CITRATE PH 4.2, 20% PEG 8000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K