4CO3
Structure of PII signaling protein GlnZ from Azospirillum brasilense in complex with adenosine triphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 |
| Unit cell lengths | 85.700, 85.700, 67.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.477 - 1.050 |
| R-factor | 0.1708 |
| Rwork | 0.159 |
| R-free | 0.19910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cny |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.737 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.480 | 1.100 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.090 | 0.880 |
| Number of reflections | 84996 | |
| <I/σ(I)> | 2.3 | 2 |
| Completeness [%] | 99.0 | 97.3 |
| Redundancy | 5.21 | 3.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M MES PH 6.5, 40% PEG 200, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
