4CO0
Structure of PII signaling protein GlnZ from Azospirillum brasilense in complex with adenosine diphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-06-24 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 |
Unit cell lengths | 86.550, 86.550, 65.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.868 - 1.400 |
R-factor | 0.2122 |
Rwork | 0.210 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cny |
RMSD bond length | 0.005 |
RMSD bond angle | 1.178 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.870 | 1.500 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.080 | 0.520 |
Number of reflections | 35124 | |
<I/σ(I)> | 12.8 | 3.2 |
Completeness [%] | 98.0 | 99.8 |
Redundancy | 5.4 | 5.33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1 MM MNCL2, 5MM 2OG, 5 MMATP, 0.2 M K/NA TARTRATE, 200% PEG 3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, pH 7.5 |