4CNY
Structure of PII signaling protein GlnZ from Azospirillum brasilense
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-05-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 87.630, 87.630, 87.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.775 - 1.200 |
| R-factor | 0.1483 |
| Rwork | 0.147 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.163 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.780 | 1.200 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.080 | 0.430 |
| Number of reflections | 44969 | |
| <I/σ(I)> | 11.8 | 3 |
| Completeness [%] | 99.1 | 96.7 |
| Redundancy | 5.59 | 3.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.4 M AMMONIUM PHOSPHATE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, pH 7.5 |
