4CNU
CRYSTAL STRUCTURE OF WT HUMAN CRMP-4 from lattice translocation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.240, 108.220, 118.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.430 - 2.800 |
| R-factor | 0.25725 |
| Rwork | 0.255 |
| R-free | 0.29484 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cns |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.981 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.240 | 1.210 |
| Number of reflections | 28951 | |
| <I/σ(I)> | 4.9 | 1.2 |
| Completeness [%] | 93.2 | 84.4 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 23% (W/V) PEG 3350, 200 MM NA TARTRATE, 100 MM BIS-TRIS PROPANE PH 7.0, 50 MM NA FLUORIDE |
