4CN1
GlgE isoform 1 from Streptomyces coelicolor D394A mutant with maltose- 1-phosphate bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-11 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 113.520, 113.520, 313.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 91.950 - 2.550 |
R-factor | 0.18616 |
Rwork | 0.185 |
R-free | 0.21507 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zss |
RMSD bond length | 0.011 |
RMSD bond angle | 1.414 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 104.530 | 2.620 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.170 | 0.660 |
Number of reflections | 67726 | |
<I/σ(I)> | 11.4 | 3 |
Completeness [%] | 99.7 | 96.6 |
Redundancy | 14 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7.0 |