4CMZ
An intertwined homodimer of the PDZ homology domain of periaxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 79.840, 79.840, 81.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.920 - 2.700 |
R-factor | 0.2049 |
Rwork | 0.203 |
R-free | 0.24100 |
Structure solution method | MIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.005 |
RMSD bond angle | 0.994 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (AUTOSOL) |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.040 | 0.780 |
Number of reflections | 15882 | |
<I/σ(I)> | 21 | 1.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.2 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 30% PEG 2000 MME, 0.15 M KBR, pH 7.5 |