4CMR
The crystal structure of novel exo-type maltose-forming amylase(Py04_0872) from Pyrococcus sp. ST04
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 291 |
Detector technology | CCD |
Collection date | 2013-06-04 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 117.639, 370.794, 82.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.130 - 1.800 |
R-factor | 0.1981 |
Rwork | 0.198 |
R-free | 0.22540 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.077 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.800 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.077 | 0.498 |
Number of reflections | 164426 | |
<I/σ(I)> | 52.6 | 6.7 |
Completeness [%] | 99.1 | 97.4 |
Redundancy | 12.8 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 1.0M AMMONIUM CITRATE DIBASIC, 0.8M SODIUM ACETATE TRIHYDRATE PH4.6 |