4CLV
Crystal Structure of dodecylphosphocholine-solubilized NccX from Cupriavidus metallidurans 31A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 53.800, 53.800, 300.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.120 |
| R-factor | 0.2517 |
| Rwork | 0.251 |
| R-free | 0.26420 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.380 | 3.340 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Rmerge | 0.060 | 1.500 |
| Number of reflections | 8629 | |
| <I/σ(I)> | 23.5 | 1.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 23.6 | 25.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 14 % PEG4000 0.1 M NA-ACETATE PH 4.5 0.1 M ZN-ACETATE |
