4CLQ
Structure of Rcl1p - Bms1p complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.120, 88.280, 102.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.570 - 2.020 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.21660 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.240 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 65101 | |
| <I/σ(I)> | 14.66 | 3.41 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 4.26 | 4.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 12 % PEG 8K, 0.2 M NACL AND 0.1 M HEPES, PH 7.5 |
