4CKP
Structure of an N-terminal fragment of Leishmania SAS-6 that contains part of its coiled coil domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 482.681, 482.681, 43.129 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.901 - 3.450 |
| R-factor | 0.2248 |
| Rwork | 0.224 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ckm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.348 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.940 | 3.570 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.340 | |
| Number of reflections | 49349 | |
| <I/σ(I)> | 8.2 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22.3 | 19.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.23 | 100 MM BISTRIS PH 6.23, 100 MM NAACETATE (NOT PH ADJUSTED), 26.5-27% (W/V) PEG-400 |
