4CKM
Structure of the N-terminal domain of Leishmania SAS-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.250, 84.250, 239.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.490 - 2.150 |
| R-factor | 0.21024 |
| Rwork | 0.208 |
| R-free | 0.24534 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.349 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.860 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.140 | 1.500 |
| Number of reflections | 48008 | |
| <I/σ(I)> | 7.6 | 1.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.1 | 100 MM BISTRIS PH 5.1, 200 MM MGCL2, 20% (W/V) PEG-3350 |
