4CJA
BurrH DNA-binding protein from Burkholderia rhizoxinica in complex with its target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.242, 95.950, 76.446 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 38.656 - 2.651 |
| R-factor | 0.2063 |
| Rwork | 0.203 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.366 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.920 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.060 | 0.610 |
| Number of reflections | 27134 | |
| <I/σ(I)> | 12.1 | 1.7 |
| Completeness [%] | 97.7 | 99.9 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
