4CJ6
Crystal structure of the complex of the Cellular Retinal Binding Protein Mutant R234W with 9-cis-retinal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-11-03 |
Detector | DECTRIS PILATUS 2M-F |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.870, 57.918, 75.206 |
Unit cell angles | 90.00, 122.88, 90.00 |
Refinement procedure
Resolution | 44.745 - 1.896 |
R-factor | 0.1683 |
Rwork | 0.165 |
R-free | 0.19750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hx3 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.841 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 2.010 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.090 | 0.340 |
Number of reflections | 19606 | |
<I/σ(I)> | 14.48 | 4.12 |
Completeness [%] | 98.4 | 92.9 |
Redundancy | 4.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |