4CIZ
Crystal structure of the complex of the Cellular Retinal Binding Protein with 9-cis-retinal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 70.556, 70.556, 299.747 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.356 - 3.403 |
| R-factor | 0.2356 |
| Rwork | 0.234 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hy5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.567 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.360 | 3.610 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.100 | 1.100 |
| Number of reflections | 6770 | |
| <I/σ(I)> | 22.68 | 2.74 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 13 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
