4CIU
Crystal structure of E. coli ClpB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-07 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 65 |
| Unit cell lengths | 127.329, 127.329, 119.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.800 - 3.500 |
| R-factor | 0.2252 |
| Rwork | 0.224 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.200 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.090 | 0.530 |
| Number of reflections | 14024 | |
| <I/σ(I)> | 16.8 | 5.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.5 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 1.5 M AMSO4, 0.4 % PEG 400, 0.1 M HEPES PH 7.5, 22 % GLYCEROL AT 291 K |
