4CIS
Structure of MutM in complex with carbocyclic 8-oxo-G containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-04 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.063, 112.692, 132.882 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.930 - 2.050 |
R-factor | 0.21507 |
Rwork | 0.214 |
R-free | 0.23885 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xc8 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.821 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.930 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.150 | 1.180 |
Number of reflections | 41439 | |
<I/σ(I)> | 10.73 | 1.5 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 5.9 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 100 MM TRIS PH 7.5, 2 MM SPERMIDINE, 1 MM TCEP, 0.01MM ZNCL2 , 90 MM LISO4, 18% PEG 4000 |