4CII
Crystal structure of N-terminally truncated Helicobacter pylori T4SS Protein CagL as domain swapped dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-24 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 61.841, 61.841, 244.499 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.282 - 2.150 |
R-factor | 0.2003 |
Rwork | 0.199 |
R-free | 0.23740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3CZI |
RMSD bond length | 0.009 |
RMSD bond angle | 0.968 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.750 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.080 | 0.500 |
Number of reflections | 16080 | |
<I/σ(I)> | 15.7 | 3.2 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 9 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.2 | 277 | 100 MM CITRATE/PHOSPHATE PH 4.2, 40% (V/V) 2-METHYL-2,4-PENTANEDIOL, 6.4% TERT-BUTANOL. VAPOR DIFFUSION AT 277 K WITH DROP RATIO OF 2:1 PROTEIN TO RESERVOIR. |