4CHK
Crystal Structure of the ARF5 oligomerization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-26 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.264, 86.438, 91.371 |
| Unit cell angles | 90.00, 112.71, 90.00 |
Refinement procedure
| Resolution | 45.260 - 2.850 |
| R-factor | 0.1843 |
| Rwork | 0.182 |
| R-free | 0.22050 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.010 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.070 | 0.750 |
| Number of reflections | 33135 | |
| <I/σ(I)> | 17.37 | 1.89 |
| Completeness [%] | 99.1 | 96.5 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 12% DIOXANE, pH 8.0 |
