4CHF
Crystal structure of the putative cap-binding domain of the PB2 subunit of Thogoto virus polymerase (form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-20 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 101.440, 101.440, 106.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.982 - 3.000 |
R-factor | 0.2004 |
Rwork | 0.200 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | THOGOTO PB2 PUTATIVE CAP-BINDING DOMAIN FORM 1 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.846 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.220 | 0.950 |
Number of reflections | 11610 | |
<I/σ(I)> | 12.9 | 3.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.2 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 100 MM BIS-TRIS PH 6.0, 100 MM MGCL2 AND 22 % PEG3350 |