4CHC
Crystal structure of the N-terminal domain of the PA subunit of Thogoto virus polymerase (form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.000, 79.160, 70.300 |
| Unit cell angles | 90.00, 97.23, 90.00 |
Refinement procedure
| Resolution | 102.180 - 2.770 |
| R-factor | 0.23127 |
| Rwork | 0.229 |
| R-free | 0.27868 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DHORI VIRUS POLYMERASE PA N-TERMINAL DOMAIN |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.070 | 0.300 |
| Number of reflections | 26711 | |
| <I/σ(I)> | 9.6 | 2.1 |
| Completeness [%] | 92.7 | 51.4 |
| Redundancy | 2.1 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MGCL2, 0.1 M MES PH 6.5 AND 22% PEG 4000 |
