4CGV
First TPR of Spaghetti (RPAP3) bound to HSP90 peptide SRMEEVD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-21 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.050, 59.120, 65.860 |
| Unit cell angles | 63.42, 67.50, 82.43 |
Refinement procedure
| Resolution | 54.832 - 2.540 |
| R-factor | 0.2416 |
| Rwork | 0.240 |
| R-free | 0.26710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE STRUCTURE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.751 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.830 | 2.610 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.100 | 0.360 |
| Number of reflections | 20092 | |
| <I/σ(I)> | 8.1 | 2 |
| Completeness [%] | 94.0 | 94.1 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
