4CGV
First TPR of Spaghetti (RPAP3) bound to HSP90 peptide SRMEEVD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-21 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 1 |
Unit cell lengths | 52.050, 59.120, 65.860 |
Unit cell angles | 63.42, 67.50, 82.43 |
Refinement procedure
Resolution | 54.832 - 2.540 |
R-factor | 0.2416 |
Rwork | 0.240 |
R-free | 0.26710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IN-HOUSE STRUCTURE |
RMSD bond length | 0.005 |
RMSD bond angle | 0.751 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.830 | 2.610 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.100 | 0.360 |
Number of reflections | 20092 | |
<I/σ(I)> | 8.1 | 2 |
Completeness [%] | 94.0 | 94.1 |
Redundancy | 2.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |