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4CGS

Crystal structure of the N-terminal domain of the PA subunit of Dhori virus polymerase

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID29
Synchrotron siteESRF
BeamlineID29
Temperature [K]100
Detector technologyPIXEL
Collection date2011-06-11
DetectorDECTRIS PILATUS 6M
Spacegroup nameP 1 21 1
Unit cell lengths43.910, 87.450, 56.130
Unit cell angles90.00, 112.98, 90.00
Refinement procedure
Resolution51.680 - 1.300
R-factor0.17509
Rwork0.173
R-free0.21964
Structure solution methodSAD
Starting model (for MR)NONE
RMSD bond length0.020
RMSD bond angle1.928
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareautoSHARP
Refinement softwareREFMAC (5.6.0116)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.350
High resolution limit [Å]1.3001.300
Rmerge0.0600.370
Number of reflections92519
<I/σ(I)>14.84.3
Completeness [%]96.694.2
Redundancy3.413.45
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
17.520MG/ML PROTEIN, 3.2 M NA FORMATE AND 0.1 M HEPES PH 7.5

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