4CGS
Crystal structure of the N-terminal domain of the PA subunit of Dhori virus polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-11 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.910, 87.450, 56.130 |
Unit cell angles | 90.00, 112.98, 90.00 |
Refinement procedure
Resolution | 51.680 - 1.300 |
R-factor | 0.17509 |
Rwork | 0.173 |
R-free | 0.21964 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.020 |
RMSD bond angle | 1.928 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.060 | 0.370 |
Number of reflections | 92519 | |
<I/σ(I)> | 14.8 | 4.3 |
Completeness [%] | 96.6 | 94.2 |
Redundancy | 3.41 | 3.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 20MG/ML PROTEIN, 3.2 M NA FORMATE AND 0.1 M HEPES PH 7.5 |